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Registro Completo |
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Biblioteca(s): |
Epagri-Sede. |
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Data corrente: |
13/12/2017 |
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Data da última atualização: |
13/12/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BOSETTO, L.; BRANCHER, T. L.; CARLESSO, C.; KVITSCHAL, M. V.; HAWERROTH, M. C. |
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Título: |
The best tissue to obtain DNA from apple tree using CTAB extraction protocol. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON SCIENCE AND BIOTECHNOLOGY, 2., 2017, VIDEIRA. Resumos... Videira: Unoesc, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The DNA extraction is the key for all molecular biology procedures. In plants, this stage is absolutely affected by the tissue compounds, that are variable between the species. In apple tree, polyphenols are the main compounds that affects DNA quality and integrity. The aim of this work was to apply a protocol for DNA extraction using different apple tree tissues and to determine the best one to obtain high DNA quality. The protocol tested was an adaptation of CTAB, using leaf, bud, stem, apple skin and flesh. The experiment was carried out in a completely randomized design with five replicates, considering each tissue. To determinate the best quality of DNA was used the relation 260 nm/ 280nm and 260nm/230nm. Quality parameters and DNA concentration were obtained with absorbance spectrophotometer. It was performed variance analysis and Tukey's test, with a significance level at 5 %. The DNA integrity was observed in agarose gel 0.8 %. The highest mean concentration were 374.53 ng/µL in stem and 395.58 ng/µL in bud tissue. The best quality (260/280 ≈ 2.0, 260/230 ≈ 1.8) was obtain with leaf, bud and stem, the same for DNA integrity. The best tissues to obtain DNA from apple tree with high concentration and quality were bud, stem and leaf, suggesting higher efficiency using this protocol. |
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Palavras-Chave: |
Biotechnology; Deoxyribonucleic acid; Malus sp; Molecular biology. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02009naa a2200217 a 4500
001 1126983
005 2017-12-13
008 2017 bl uuuu u00u1 u #d
100 1 $aBOSETTO, L.
245 $aThe best tissue to obtain DNA from apple tree using CTAB extraction protocol.$h[electronic resource]
260 $c2017
520 $aThe DNA extraction is the key for all molecular biology procedures. In plants, this stage is absolutely affected by the tissue compounds, that are variable between the species. In apple tree, polyphenols are the main compounds that affects DNA quality and integrity. The aim of this work was to apply a protocol for DNA extraction using different apple tree tissues and to determine the best one to obtain high DNA quality. The protocol tested was an adaptation of CTAB, using leaf, bud, stem, apple skin and flesh. The experiment was carried out in a completely randomized design with five replicates, considering each tissue. To determinate the best quality of DNA was used the relation 260 nm/ 280nm and 260nm/230nm. Quality parameters and DNA concentration were obtained with absorbance spectrophotometer. It was performed variance analysis and Tukey's test, with a significance level at 5 %. The DNA integrity was observed in agarose gel 0.8 %. The highest mean concentration were 374.53 ng/µL in stem and 395.58 ng/µL in bud tissue. The best quality (260/280 ≈ 2.0, 260/230 ≈ 1.8) was obtain with leaf, bud and stem, the same for DNA integrity. The best tissues to obtain DNA from apple tree with high concentration and quality were bud, stem and leaf, suggesting higher efficiency using this protocol.
653 $aBiotechnology
653 $aDeoxyribonucleic acid
653 $aMalus sp
653 $aMolecular biology
700 1 $aBRANCHER, T. L.
700 1 $aCARLESSO, C.
700 1 $aKVITSCHAL, M. V.
700 1 $aHAWERROTH, M. C.
773 $tIn: INTERNATIONAL SYMPOSIUM ON SCIENCE AND BIOTECHNOLOGY, 2., 2017, VIDEIRA. Resumos... Videira: Unoesc, 2017.
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| 84. |  | SILVA, M. C. C. R.; CASTILHOS, R. V.; NESI, C. N.; SILVA, L. R. S.; BENTO, J. M. S. Dalbulus maidis, the insect vector of Maize rayado fino virus (MRFV), does not distinguish between two maize genotypes in field conditions but seems to prefer asymptomatic plants. In: WORKSHOP BRASILEIRO DE EPIDEMIOLOGIA DE DOENÇAS DE PLANTAS, 6., 2022, Chapecó. Resumos... Brasília: Sociedade Brasileira de Fitopatologia, 2022. p. 43| Tipo: Resumo em Anais de Congresso |
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| 85. | | SILVA, P. R. A.; ARAUJO, L.; RESENDE, R. S.; MAFFIA, L. A.; EINHARDT, A. M.; OLIVEIRA, H. R.; ALVES, Y. H.; SILVA, L. F.; RODRIGUES, F. Á. Silicon, Clonostachys rosea, and their interaction for gray mold management in cucumber. Journal of Plant Pathology, Switzerland, p. 1-8, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
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| 86. | | SOUZA, M. L. R.; CASACA, J. de M.; SILVA, L. O. da; GANECO, L. N.; NAKAGHI, L. S.; FARIA, H. S. A.; SCHMIDT, T. A.; FRANCO, N. do P. Resistencia da pele de carpa espelho (Cyprinus carpio specularis) curtida pelas tecnicas ao cromo e bioleather. Acta Scientiarum: Animal Sciences, Maringa, v. 26, n. 4, p. 421-427, 2004.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
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| 87. | | SOUZA, M. L. R.; CASACA, J. M.; NAKAGHI, L. S. O.; FRANCO, N. P.; SILVA, L. O.; VIEGAS, E. M. M. Efeito da técnica de curtimento e do método utilizado para remoção da pele da tilápia do nilo sobre as características de resistência do couro. Revista Brasileira de Zootecnia, Sociedade Brasileira de Zootecnia, v. 35, n. 4, p. 1273-1280, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
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| 88. | | SOUZA, M. L. R.; VISENTAINER, J. V.; SOUZA, N. E.; CASACA, J. M.; SILVA, L. O.; FRANCO, N. P.; GODOY, L. C.; DOURADO, D. M. Comparação da resistência do couro da tilápia do Nilo, pacu e carpa prateada para aplicação na indústria coureira Revista Aquicultura & Pesca, Dipemar, v. 1, n. 8, p. 10-18, 2005.| Tipo: Artigo de Divulgação na Mídia |
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| 89. | | THUNG, M.; FERREIRA, R. M.; MIRANDA, P.; MODA-CIRINO, V.; FERRÃO, M. G.; SILVA, L.; DOURADO, V.; HEMP, S.; SOUZA, B.; SERPA, E.; ZIMMERMANN, M.; SINGH, S. Performance in Brazil and Colombia of common bean lines from the second selection cycle. Rev. Brasil. Genét. (Brazil. J. Genetics), Ribeirão Preto, SP, v. 16, n. 1, p. 115-127, 1993.| Biblioteca(s): Epagri-Sede. |
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| 92. | | VILLA, F.; OLIVEIRA, A. F.; SILVA, D. F.; VIEIRA NETO, J.; SILVA, L. F. O. Déficit hídrico e paclobutrazol no crescimento inicial de plantas jovens de oliveira. Cultivando o Saber, Cascavel, v. 5, n. 4, p. 38-51, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - -- |
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